Transcription plasticity in cancer
INSTITUT
Nom : IRCM – Institut de Recherche en Cancérologie de Montpellier
Adresse : 208 rue des Apothicaires 34298 Montpellier Cedex 5 FRANCE
Téléphone : 0411283177
Email : priyanka.sharma@inserm.fr
TUTEUR
Nom : Priyanka Sharma
Fonction : INSERM-CRCN
Téléphone : 0411283177
Email : priyanka.sharma@inserm.fr
STAGE
Sujet :
Transcription plasticity in cancer
Description courte :
We are fascinated by one of the posttranslational modifications, known as Arginine citrullination to investigate its potential function in transcriptional plasticity in cancer progression. Arginine citrullination is the post-translational modification of arginine to the non-coded amino acid citrulline, catalyzed by a family of enzymes called peptidyl arginine deiminases (PADIs). PADI2 is widely expressed among the family members and regulates several cellular processes associated with tumor progression. PADI2 is intricately involved in the progression of several tumors while the underlying functional mechanism could differ from one malignancy to another. Our work spotlights the PADI2-mediated citrullination of the arginine1810 Cit1810 of RNA polymerase II (RNAP2) as a key player in the transcription plasticity of breast cancer cells. Now, we are aiming to understand the functional implications of citrullination in steroid hormone regulation. Towards this goal, the potential candidate will elucidate the function of citrullination in transcription plasticity in breast cancer progression.
Activité :
Depending on the motivation of the potential candidate, we will use molecular biology and genomics tools to understand the role of protein citrullination in breast cancer progression.
Compétences requises :
The selected candidate will acquire fundamental skills for breast cancer cell lines. Also, she/ he will be able to learn to generate breast cancer organoids derived from single cells. The candidate will be benefitted by working on an international project using advanced genomics, cell biology, and molecular biology tools.
Encadrement :
Highly motivated candidates could send me a CV, motivation letter, and references letter from at least two referees to me by email on priyanka.sharma@inserm.fr. I will be happy to arrange the first virtual meeting for a short discussion followed by in-person meetings at our lab, IRCM, Montpellier
Gratification de stage :
Sur ressources propres de l’équipe d’accueil
Tutelle de stage :
From our Team at IRCM
